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目的观察肝X受体(liver-X-receptors,LXRs)对转化生长因子β1(TGF-β1)生物功能的影响,并探讨其可能的机制。方法将培养的人气道上皮细胞分为对照组、TGF-β1组、LXRs低剂量组、LXRs中剂量组和LXRs高剂量组,LXRs组分别予2.5 mol/L、5 mol/L和10 mol/L的LXRs共孵育2 h后,与TGF-β1组一起加TGF-β1反应。2 h后用Real-time PCR方法检测各组细胞纤溶酶原激活物抑制剂1(PAI-1)的mRNA表达,在反应30 min时用免疫荧光法检测P-Smad2的核转位情况。结果 TGF-β1组PAI-1 mRNA表达较对照组明显升高,差异有统计学意义(P<0.05),LXRs组PAI-1 mRNA表达较TGF-β1组明显降低,差异有统计学意义(P<0.05),LXRs剂量越大,降低越明显,予LXRs 10 mol/L干预PAI-1的mRNA下降最明显。对照组、TGF-β1组、LXRs高剂量组P-Smad2阳性细胞百分比分别为(1.752±0.423)%、(95.060±1.854)%、(1.941±0.409)%,对照组与TGF-β1组比较差异有统计学意义(P<0.05),TGF-β1组与LXRs高剂量组比较差异有统计学意义(P<0.05)。结论 LXRs可能通过减少核内P-Smad2含量来影响TGF-β1的生物学功能。
Objective To observe the effect of liver-X-receptors (LXRs) on the biological function of transforming growth factor β1 (TGF-β1) and to explore its possible mechanism. Methods The cultured human airway epithelial cells were divided into control group, TGF-β1 group, LXRs low dose group, LXRs medium dose group and LXRs high dose group, LXRs group were treated with 2.5 mol / L, 5 mol / L and 10 mol / L LXRs co-incubated for 2 h, with TGF-β1 group plus TGF-β1 response. The expression of PAI-1 mRNA in each group was detected by Real-time PCR 2 h later. The nuclear translocation of P-Smad2 was detected by immunofluorescence at 30 min after reaction. Results The expression of PAI-1 mRNA in TGF-β1 group was significantly higher than that in control group (P <0.05), and the expression of PAI-1 mRNA in LXRs group was significantly lower than that in TGF-β1 group <0.05). The larger the dose of LXRs, the more obvious the decrease was. The decrease of PAI-1 mRNA was the most obvious when LXRs 10 mol / L was added. The percentage of P-Smad2 positive cells in high-dose group and TGF-β1 group was (1.752 ± 0.423)%, (95.060 ± 1.854)% and (1.941 ± 0.409)%, respectively (P <0.05). The difference between TGF-β1 group and LXRs high-dose group was statistically significant (P <0.05). Conclusion LXRs may affect the biological function of TGF-β1 by decreasing the content of P-Smad2 in the nucleus.