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从病死栉孔扇贝 (Chlamysfarreri)体内分离到多株特殊细菌。革兰氏染色阴性 ;电镜观察为圆形、近似圆形 ,最小为 5 0nm左右 ,最大可达 4 0 0 0nm以上。该细菌在人工培养基上形不成肉眼可见菌落 ,倒置显微镜下其菌落为无色透明、圆形、小点状菌落。解剖显微镜下挑取单个菌落进行纯培养。以 0 .2 0 μm微孔滤膜抽滤细菌培养物 ,将滤液接种含 2 .2 %NaCl脑心浸液 ,生长出的细菌与上面描述的细菌完全一致。将细菌培养液以 0 .2 0 μm微孔滤膜抽滤后 15 0 0 0rpm离心2 0min浓缩细菌 ,提取核酸并检测对RNA酶敏感性 ,确定其核酸对RNA酶敏感 ,因此确定其核酸以RNA为主 ,将其确定为RNA细菌繁殖体。其分类地位尚待进一步确定。
Several special bacteria were isolated from the chlamys farreri. Gram stain negative; electron microscopy is circular, approximately circular, the minimum is about 5 0nm, up to 4 0 0 0 nm or more. The bacteria in the artificial medium can not form a macroscopic colonies, the inverted microscope under a colorless, transparent, round, dot-shaped colonies. Single colonies were picked under a dissecting microscope for pure culture. The bacterial cultures were aspirated through a 0.2 μm membrane filter and inoculated with 2.2% NaCl brain heart infusion. The resulting bacteria were identical to those described above. The bacteria culture medium was filtered with 0 2 0 μm microporous membrane filter, then centrifuged at 15 0 0 0 rpm for 2 0 min to concentrate the bacteria. The nucleic acid was extracted and the sensitivity to RNAse was determined to confirm that the nucleic acid was sensitive to RNAse. Therefore, RNA-based, identified as RNA bacterial multiplication. Its classification status needs to be further confirmed.