论文部分内容阅读
应用ＥＬＩＳＡ双抗体夹心法检测尿路感染（ＵＴＩ）患者血清和尿ｓＩＬ－２Ｒ水平。结果显示：①膀胱炎患者血清和尿ｓＩＬ－２Ｒ水平显著低于健康人（Ｐ＜０．０５），提示免疫细胞功能低下可能是ＵＴＩ的易感因素之一。②急性肾盂肾炎（ＡＰＮ）患者血清和尿ｓＩＬ－２Ｒ水平显著高于健康人（Ｐ＜０．０５），提示Ｔ细胞参与了ＡＰＮ机体防御。③慢性肾盂肾炎（ＣＰＮ）患者血清ｓＩＬ－２Ｒ水平显著高于ＡＰＮ组和健康人（分别为Ｐ＜０．０５，Ｐ＜０．０１），尿ｓＩＬ－２Ｒ水平显著高于健康人（Ｐ＜０．００１）且与尿ＩＬ－８水平呈显著正相关（ｎ＝１８，ｒ＝０．５３６，Ｐ＜０．０５），提示Ｔ细胞在ＣＰＮ机体防御中起重要作用，同时局部免疫功能紊乱可能是炎症持续进展的原因之一。④血清和尿ｓＩＬ－２Ｒ检测对肾盂肾炎与膀胱炎鉴别可能有参考价值。
Serum and urinary sIL-2R levels in patients with urinary tract infection (UTI) were detected by ELISA double antibody sandwich method. The results showed that: (1) Serum and urinary sIL-2R levels in patients with cystitis were significantly lower than those in healthy people (P <0.05), suggesting that immune cell dysfunction may be one of the predisposing factors for UTI. ② Serum and urinary sIL-2R levels in patients with acute pyelonephritis (APN) were significantly higher than those in healthy people (P <0.05), suggesting that T cells are involved in the defense of APN. ③ The level of serum sIL-2R in patients with chronic pyelonephritis (CPN) was significantly higher than that in APN and healthy subjects (P <0.05, P <0.01, respectively) 0.001), and there was a significant positive correlation with urinary IL-8 level (n = 18, r = 0.536, P <0.05), suggesting that T cells play an important role in the defense of CPN and local immune dysfunction May be one of the reasons for the continued progress of inflammation. ④ serum and urine sIL-2R detection of pyelonephritis and cystitis may have reference value.