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根据ＧＡＭｃＤＮＡ所编码的氨基酸序列合成了ＧＡＭ分子羧基端２３个氨基酸的多肽，并利用ＧＭＢＳ双功能试剂将人工合成多肽成功地与ＯＶＡ进行了交联，免疫家兔制备了抗ＧＡＭ合成多肽的抗体，并经亲和层析进行了纯化。对此抗体进行鉴定的结果表明，抗ＧＡＭ合成多肽的抗体可与天然ＧＡＭ分子发生特异性反应，并可用于免疫沉淀和Ｗｅｓｔｅｒｎｂｌｏｔ，为ＧＡＭ分子功能的研究提供了有力的工具。
According to the amino acid sequence encoded by GAMcDNA, a polypeptide of 23 amino acids at the carboxyl terminus of GAM molecule was synthesized, and the synthetic peptide was successfully cross-linked with OVA using GMBS bifunctional reagent. Anti-GAM antibody was prepared by immunizing rabbits. And purified by affinity chromatography. The results showed that the anti-GAM antibody could react specifically with native GAM and could be used in immunoprecipitation and Western blotting, which provided a powerful tool for studying the function of GAM.