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1994年从北京腹泻儿童中鉴定的国内首例G9型A组轮状病毒(Group A Rotavirus,RVA)株T203属于G9-Ⅵ(VP7基因进化树支Ⅵ),而之后国内流行的G9型RVAs主要属于全球广泛流行的G9-Ⅲ(VP7基因进化树支Ⅲ)。本研究于2010年通过基因测序从北京腹泻儿童中再次鉴定了一例RVA G9-Ⅵ。为了解北京儿童中G9-Ⅵ再次流行的情况,本研究对国内外人G9型RVAs的VP7基因进行多序列比对分析,在G9-Ⅲ和G9-Ⅵ病毒各自VP7基因序列比较保守、彼此间变异集中的区域设计杂交探针,经PCR合成地高辛标记的cDNA探针,建立区分G9-Ⅲ和G9-Ⅵ的斑点杂交方法;结合本实验室前期建立的RVA常见G和P基因型杂交鉴定方法,对2011-2012年门诊腹泻就诊患儿中RVAs的流行进行调查。结果显示G9型检出率最高(43.5%,57/131),其次是G3(30.5%)、G1(12.2%)和G2(11.5%),未发现G4型。P分型结果显示P[8]为主要基因型(85.2%),其次P[4]型(14.8%),未发现P[6]型。对57例G9型RVAs全部进行G9-Ⅲ和G9-Ⅵ杂交鉴定。结果显示:G9-Ⅵ占96.5%,取代G9-Ⅲ(3.5%)成为优势流行的G9型病毒。从VP7基因进化树图上看到,本研究鉴定的北京人RVA G9-Ⅵ同国内外近期多地出现的人RVA G9-Ⅵ进化关系密切,并且和加拿大的猪RVA株F7P4进化距离近。国内外新近重新出现的人G9-Ⅵ RVAs是否在进化过程中获得了比以往广泛流行的RVA G9-Ⅲ更强的传播能力或致病力,值得进一步研究。
The first case of Group A Rotavirus G20 (RVA) strain T203 from Beijing diarrhea children in 1994 belonged to G9-Ⅵ (VP7 gene phylogenetic tree branch Ⅵ), while the most popular type G9 RVAs in China were mainly It belongs to G9-Ⅲ (VP7 gene evolution tree branch) widely prevailed in the world. In this study, one case of RVA G9-Ⅵ was identified from diarrhea children in Beijing by genetic sequencing in 2010. To understand the prevalence of G9-Ⅵ in Beijing children, this study analyzed the VP7 gene of G9-type RVAs at home and abroad by multiple sequence alignment. The VP7 gene sequences of G9-Ⅲ and G9-Ⅵ viruses were relatively conservative and mutated In order to establish a dot hybridization method that distinguishes G9-Ⅲ and G9-Ⅵ by PCR amplification of cDNA probes labeled with digoxigenin, Method to investigate the prevalence of RVAs in pediatric diarrhea clinic during 2011-2012. The results showed that the detection rate of G9 was the highest (43.5%, 57/131), followed by G3 (30.5%), G1 (12.2%) and G2 (11.5%). No G4 was found. P typing showed that P [8] was the major genotype (85.2%), followed by P [4] (14.8%) and no P [6]. All 57 G9 RVAs were identified by G9-Ⅲ and G9-Ⅵ hybridization. The results showed that: G9-Ⅵ accounted for 96.5%, instead of G9-Ⅲ (3.5%) became the predominant prevalent G9 virus. The VP7 gene phylogenetic tree shows that the RVA G9-Ⅵ identified in this study is closely related to the recent emergence of human RVA G9-Ⅵ at home and abroad, and close to that of the Canadian pig RVA strain F7P4. Whether human G9-Ⅵ RVAs, newly emerging at home and abroad, gained stronger transmissibility or virulence than RVA G9-Ⅲ, which is widely prevalent in the world, deserved further study.