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目的检测腹腔镜结直肠癌根治术患者外周静脉血CK20 mRNA的表达,探讨腹腔镜结直肠癌根治术对术后肿瘤细胞血循环微转移的影响。方法应用FQ-PCR方法检测24例行腹腔镜辅助下结直肠癌根治术患者(腹腔镜组)和31例传统开腹结直肠癌根治术患者(开腹组)外周静脉血CK20 mRNA表达的变化。结果腹腔镜组CK20 mRNA术前阳性率为29.17%(7/24),术后阳性率为37.50%(9/24),两者之间无显著性差异(P>0.05);腹腔镜组和开腹组手术前后比较,也无显著差异(P>0.05)。结论腹腔镜结直肠癌根治术和传统开腹手术一样,并不增加外周静脉血CK20 mRNA的阳性率,即不增加术后肿瘤血循环微转移的危险性。
Objective To detect the expression of CK20 mRNA in peripheral blood of patients undergoing laparoscopic radical resection of colorectal cancer and to investigate the effect of laparoscopic radical resection on the micrometastasis of tumor cells. Methods FQ-PCR was used to detect the expression of CK20 mRNA in peripheral blood of 24 patients undergoing laparoscopic-assisted radical resection of colorectal cancer (laparoscopic group) and 31 patients undergoing open radical resection of colorectal cancer (open group) . Results The preoperative positive rate of CK20 mRNA in laparoscopic group was 29.17% (7/24), postoperative positive rate was 37.50% (9/24), there was no significant difference between the two groups (P> 0.05) Before and after laparotomy, there was no significant difference (P> 0.05). Conclusions Laparoscopic radical resection of colorectal carcinoma, like conventional laparotomy, does not increase the positive rate of CK20 mRNA in peripheral venous blood, that is, it does not increase the risk of postoperative tumor micrometastasis.