论文部分内容阅读
1 材料和方法受精15天人胚一例,取自妊娠合并子宫肌瘤切除术患者,剖开子宫腔,宫体侧壁可见直径约1.2cm的囊状隆起,剪开囊壁,早期胚位于囊内,呈白色细线状.结合末次月经日期,测量胚长度2mm,固定于10%甲醛溶液中,石蜡包埋,整体连续切片厚5μm,HE染色,光镜观察.2 结果15天人胚绒毛膜主要由细胞滋养层和合体滋养层细胞组成,少数绒毛中可见胚外中胚层,个别绒毛中轴内可见内皮细胞围成的毛细血管,血管腔内未见血细胞(图1).此时,三胚层胚盘已形成(图2).内胚层细胞呈立方状,核圆,清晰可辨,其边缘与卵黄囊相连.卵黄囊壁内侧由单层立方上皮围成,近腔面偶见圆球形,体积较大,嗜酸性染色的原始生殖细胞;壁外侧紧贴单层立方上皮可见由胚外中胚层分化的单层,近似于扁平
MATERIALS AND METHODS One case of fertilized human embryos of 15 days was taken from patients with pregnancy and uterine myoma excision. The uterine cavity was dissected. The cystoid wall with a diameter of about 1.2 cm was visible and the cyst wall was cut. The early embryo was located in the sac With a white thin line shape.Combined with the last menstrual date, the length of the embryo was measured 2mm, fixed in 10% formalin solution, embedded in paraffin, the overall serial thickness 5μm, HE staining, light microscopy.2 results 15 days human embryo villi Membrane mainly by the trophoblast and syncytiotrophoblast cells, a small number of villi can be seen in the mesoderm, endothelial cells within the individual villi can be seen surrounded by capillaries, blood vessels within the lumen (Figure 1) .At this time, Three embryonic germ plate has been formed (Figure 2). Endoderm cells were cubic, nuclear round, clearly visible, the edge of the yolk sac connected to the inside of the yolk wall surrounded by a single layer of cubic epithelium, Spherical, bulky, eosinophilic staining of primordial germ cells; close to the outer wall of the monolayer of cubic epithelium visible from the mesoderm differentiation of the monolayer, similar to the flat