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木瓜蛋白酶作为外用药,可水解坏死组织,但有副作用。固定化木瓜蛋白酶涂膜剂是由虾壳中提取的壳聚糖与戊二醛交联、固定化制备出来的。0.4%的戊二醛与1%中等粘度壳聚糖(η=100~250MPa·s)经摇床振荡(200r/min,2h)充分交联,加木瓜蛋白酶液(2.09mg/mL,pH7.8)4℃固定12h,涂抹皮肤自然成膜。此固定化涂膜剂最适温度55℃,最适pH7.8,最大载酶量为45mg/g载体,酶相对活力30~35%。与未固定化木瓜蛋白溶液酶相比,提高了其对热、乙醇、脲的稳定性,将改善溶液木瓜蛋白酶药用时的副作用
Papain as an external use, can hydrolyze necrotic tissue, but there are side effects. Immobilized papain coating agent is extracted from the shrimp shell chitosan cross-linked with glutaraldehyde, prepared by the immobilization. 0.4% of glutaraldehyde and 1% of medium viscosity chitosan (η = 100 ~ 250MPa · s) were shaken by shaking (200r / min, 2h) fully crosslinked, plus papain solution , PH7.8) 4 ℃ fixed 12h, smear the skin natural film. The optimal coating temperature 55 ℃, the optimum pH7.8, the maximum amount of enzyme carrying 45mg / g carrier, enzyme relative activity of 30 to 35%. Compared with un-immobilized papain solution enzyme, its stability to heat, ethanol and urea is improved, which will improve the side effects of solution papain when used medicinally