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目的通过研究脑蛋白水解物注射液(CL)对体外培养神经祖细胞(NPCs)增殖和分化的影响,探讨其改善阿尔茨海默病认知功能的机理。方法从成年大鼠脑不同区域原代及传代培养神经祖细胞,以乳酸脱氢酶(LDH)分析法测定细胞活性,以免疫荧光染色法鉴定细胞性质,以蛋白印迹转移(Western blot)法检测蛋白质表达变化。结果CL处置明显增加神经祖细胞数及MAP2a/b、SynapsinⅠ表达水平。结论CL促进神经祖细胞增殖和向神经元分化,可能是其改善阿尔茨海默病认知功能的机理之一。
Objective To study the effect of brain protein hydrolyzate injection (CL) on the proliferation and differentiation of neural progenitor cells (NPCs) in vitro and to explore its mechanism of improving cognitive function in Alzheimer’s disease. Methods Primary and subcultured neural progenitor cells were cultured in different regions of adult rat brain. The cell viability was determined by lactate dehydrogenase (LDH) assay. The cell characteristics were identified by immunofluorescence staining and Western blotting Changes in protein expression. Results CL treatment significantly increased the number of neural progenitor cells and MAP2a / b, Synapsin Ⅰ expression levels. Conclusions CL can promote the proliferation of neural progenitor cells and differentiate into neurons, which may be one of the mechanisms that improve the cognitive function of Alzheimer’s disease.