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目的预测各型丙型肝炎病毒(HCV)的MHC-Ⅱ类抗原表位,为HCV的疫苗研发提供理论依据。方法从NCBI数据库中检索出10条包含6种基因型的HCV完整氨基酸序列,使用在线MHC-Ⅱ类抗原表位分析工具NetMHCⅡpan-2.1 Server,从MHC-Ⅱ类抗原表位在6种HCV型别之间的保守性及与MHC-Ⅱ类分子亲和力两方面评价HCV携带的潜在MHC-Ⅱ类抗原表位。结果位于NS3和NS5B蛋白的3段表位核心序列具有较好的保守性,其中尤以NS3蛋白携带的表位核心序列保守性最好;高亲和力的MHC-Ⅱ类抗原表位主要位于E2、NS5A、NS4A和NS5B蛋白,其中E2和NS5A蛋白携带的高亲和力抗原表位较为丰富。结论 NS5B蛋白所携带的MHC-Ⅱ类抗原表位兼顾了各个HCV基因型之间的保守性及与MHC-Ⅱ类分子的高亲和力,具有较好的疫苗应用前景;NS3、E2、NS5A和NS4A蛋白有可能成为HCV多表位肽疫苗的备选组分。
Objective To predict the MHC class Ⅱ epitopes of various types of hepatitis C virus (HCV) and provide a theoretical basis for the development of vaccine against HCV. Methods A total of 10 complete amino acid sequences of HCV including 6 genotypes were retrieved from the NCBI database. NetMHC Ⅱ pan-2.1 Server, an on-line MHC class Ⅱ antigen epitope analysis tool, was used to amplify MHC-Ⅱ epitopes from 6 HCV genotypes And the potential MHC-Ⅱ epitopes that HCV carries in both aspects of MHC-Ⅱ molecule affinity. Results The core sequences of NS3 and NS5B epitopes were well conserved. Among them, NS3 protein had the best conserved core sequence. The high affinity MHC-Ⅱ epitope was mainly located in E2, NS5A, NS4A and NS5B proteins, E2 and NS5A protein carrying high affinity epitopes are abundant. Conclusion The epitopes of MHC-Ⅱ carried by NS5B protein take into consideration the conservatism of HCV genotypes and the high affinity with MHC-Ⅱ molecules, which has a good prospect of vaccine application. NS3, E2, NS5A and NS4A The protein is likely to be an alternative component of the HCV multi-epitope peptide vaccine.