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我们的前期研究发现,中药单体淫羊藿甙(ICA)具有一定的抗肿瘤作用,是一种新型的生物反应调节剂和诱导分化剂.本实验中,我们选用淫羊藿甙作用于人高转移肺癌细胞PG,采用多件检测手段对肿瘤细胞恶性表型逆转的多个方面进行了较深入的研究,进一步揭示淫羊藿甙的抗肿瘤作用及其调控机制.分别采用MTT法、放免法、流式细胞术、粘附实验及运动、侵袭实验检测了ICA处理后PG细胞增殖能力,细胞内cAMP,cGMP水平,细胞周期,细胞的粘附性及运动、侵袭能力等方面的变化.结果发现,1.与对照组相比,200μg/mlICA对PG细胞有较弱的增殖抑制作用;2.与对照组相比,200μg/mlICA作用于PG细胞24h后,细胞内cAMP水平升高(P<0.05),cGMP水平降低(P<0.01),cAMP/cGMP比值明显升高(P<0.01);3.流式细胞仪检测扫描图显示,与对照组相比,ICA处理24h后,G0/G1期细胞增多,而S期
Our previous study found that Chinese herbal medicine Epimedium (ICA) has a certain anti-tumor effect and is a novel biological response modifier and inducer. In this experiment, we used epimedium to act on humans. Highly metastatic lung cancer cell PG, using multiple detection methods to conduct a more in-depth study of multiple aspects of tumor cell malignant phenotype reversal, and further reveal the anti-tumor effect of epimedium and its regulatory mechanisms. MTT method, the use of radiation The proliferative capacity of PG cells, intracellular cAMP, cGMP levels, cell cycle, cell adhesion, and movement and invasive ability of PG cells were detected by flow cytometry, flow cytometry, adhesion assay, and exercise and invasion assay. The results showed that 1. Compared with the control group, 200μg/ml ICA had a weaker inhibitory effect on PG cells; 2. Compared with the control group, 200μg/ml ICA acted on the PG cells for 24h, and the intracellular cAMP level was increased ( P<0.05), cGMP levels decreased (P<0.01), cAMP/cGMP ratio significantly increased (P<0.01); 3. Flow cytometry scans showed that compared with the control group, ICA treatment after 24h, G0 Increased number of cells in /G1 phase, and S phase