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目的 探讨氧化苦参碱 (Oxy)对肺癌和胃癌细胞诱导血管内皮细胞 (VEC)增殖的抑制作用。方法 用MTT法检测不同浓度 Oxy对 VEC、人肺腺癌 SPC- A - 1细胞、人低分化胃癌 MKN- 45细胞增殖及 SPC- A- 1和MKN- 45细胞诱导 VEC增殖的抑制。结果 Oxy浓度为 2 .5～ 10 m g/ ml时 ,对 VEC增殖的抑制率为 4.6 %～36 .4% ;Oxy浓度为 0 .15 6～ 10 mg/ m l时 ,对肺癌 SPC- A- 1和胃癌 MKN- 45细胞增殖的抑制率分别为 3.7%～93.9%和 2 .1%～ 93.6 % ;经浓度为 1.2 5～ 10 m g/ ml Oxy作用后的肺癌 SPC- A - 1和胃癌 MKN- 45细胞条件培养液对 VEC增殖的抑制率分别为 11.1%～ 37.0 %和 8.7%～ 39.1% ;Oxy浓度为 1.2 5～ 10 mg/ ml时 ,对肺癌 SPC-A- 1和胃癌 MKN- 45细胞条件培养液诱导 VEC增殖的抑制率分别为 13.8%～ 5 8.6 %和 15 .9%～ 79.4%。结论 Oxy对肺癌和胃癌细胞诱导的 VEC增殖具有抑制作用。
Objective To investigate the inhibitory effect of oxymatrine (Oxy) on the proliferation of vascular endothelial cells (VEC) induced by lung cancer and gastric cancer cells. Methods The proliferation of VEC, human lung adenocarcinoma SPC- A-1 cells, and human poorly differentiated gastric cancer MKN-45 cells and the inhibition of VEC proliferation induced by SPC-A-1 and MKN-45 cells were detected by MTT assay. Results When the Oxy concentration was 2.5 to 10 mg/ml, the inhibition rate of VEC proliferation was 4.6% to 36.4%; when the Oxy concentration was 0.16 to 10 mg/ml, SPC-A-1 was applied to lung cancer. The inhibition rates of proliferation of gastric cancer and MKN-45 cells were 3.7%-93.9% and 2.1%-93.6%, respectively; lung cancer SPC-A-1 and gastric cancer MKN- after 1.25-15-10 mg/ml Oxy. The inhibitory rate of VEC proliferation in the 45-cell conditioned medium was 11.1%-37.0% and 8.7%-39.1%, respectively; when the Oxy concentration was 1.2-5-10 mg/ml, the lung cancer SPC-A-1 and gastric cancer MKN-45 cells The inhibitory rate of VEC proliferation induced by conditioned medium was 13.8% to 58.6% and 15.9% to 79.4%, respectively. Conclusion Oxy can inhibit the proliferation of VEC induced by lung cancer and gastric cancer cells.