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目的观察丹参川芎嗪对乙型肝炎大鼠病理形态学及TNF-α、TGF-β1表达影响。方法选择雄性SD大鼠,随机分为对照组(A)、模型组(B)和丹参川芎嗪组(C),每组10只;静脉注射乙型肝炎病毒抗原复制肝炎模型,C组腹腔注射丹参川芎嗪,连续给药3周;免疫组化法检测肝组织TNF-α、TGF-β1表达,ELISA法测定血清TNF-α、TGF-β1、HA、PCⅢ、LN,HE染色观察肝组织病理形态。结果与A组比较,B组ALT、AST、HA、PCⅢ、LN、TNF-α、TGF-β1明显升高;TNF-α、TGF-β1表达增加,肝组织病理损伤明显;C组ALT、AST、HA、PCⅢ、LN、TNF-α、TGF-β1明显低于B组;TNF-α、TGF-β1表达降低,肝组织病理损伤显著改善,上述差异均有统计学意义(P<0.05)。结论丹参川芎嗪可改善乙型肝炎大鼠肝纤维化程度,其作用可能与下调TNF-α、TGF-β1表达有关。
Objective To observe the effect of ligustrazine tetramethylpyrazine on the pathomorphology and the expression of TNF-α and TGF-β1 in Hepatitis B rats. Methods Male Sprague-Dawley rats were randomly divided into control group (A), model group (B) and ligustrazine group (C), with 10 rats in each group. Hepatitis B virus was injected intravenously into hepatitis B virus Salviae miltiorrhizae and ligustrazine were administrated continuously for 3 weeks. The expression of TNF-α and TGF-β1 in the liver tissues were detected by immunohistochemistry and the levels of TNF-α, TGF-β1, HA, PCⅢ, form. Results Compared with group A, the levels of ALT, AST, HA, PCⅢ, LN, TNF-α and TGF-β1 in group B were significantly increased, while the expressions of TNF-α and TGF-β1 were increased The levels of TNF-α, TGF-β1, HA, PCⅢ, LN, TNF-α and TGF-β1 were significantly lower than those in group B. The expressions of TNF-α and TGF-β1 were decreased and the pathological changes of liver tissue were significantly improved (all P <0.05). Conclusion Salviae miltiorrhizae can improve hepatic fibrosis in rats with hepatitis B, which may be related to the down-regulation of the expression of TNF-α and TGF-β1.