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目的:探讨消癌解毒方含药血清对人肝癌细胞SMMC-7721细胞端粒酶及凋亡相关基因mRNA表达的影响。方法:消癌解毒方含药血清作用于人肝癌SMMC-7721细胞,ELISA试剂盒检测各组端粒酶浓度,采用实时荧光定量PCR方法检测药物作用前后各组SMMC-7721细胞Bax、Bcl-2、CytC、Caspase-3 mRNA表达水平。结果:消癌解毒方含药血清作用于人肝癌SMMC-7721细胞可以降低其端粒酶浓度,且呈剂量依赖性;可使Bcl-2 mRNA表达水平下调,使Bax、CytC、Caspase-3 mRNA表达水平上调,下调Bcl-2/Bax mRNA比例,且中、高剂量组与对照组比较效果明显(P<0.01,P<0.05)。结论:消癌解毒方含药血清可能通过抑制端粒酶活性,调控凋亡相关基因mRNA的表达,抑制人肝癌SMMC-7721细胞生长,促使其凋亡,在肝癌治疗中发挥疗效。
Objective: To investigate the effect of Xiaoyan Jiedu Fang containing serum on the mRNA expression of telomerase and apoptosis related genes in human hepatoma SMMC-7721 cells. Methods: The drug-containing serum of Xiaoyao Jiedu Decoction was applied to human hepatocellular carcinoma SMMC-7721 cells. The concentration of telomerase in each group was detected by ELISA kit. The levels of Bax and Bcl-2 in SMMC-7721 cells were detected by real-time fluorescence quantitative PCR , CytC, Caspase-3 mRNA expression levels. Results: The drug-containing serum of Xiaoyao Jiedu Decoction decreased the telomerase concentration in a dose-dependent manner in human hepatocellular carcinoma SMMC-7721 cells, down-regulated the expression of Bax, CytC and Caspase-3 mRNA The expression of Bcl-2 / Bax mRNA was down-regulated in the medium and high dose groups compared with the control group (P <0.01, P <0.05). Conclusion: The serum of Xiaoyao Jiedu Decoction may inhibit the telomerase activity, regulate the expression of apoptosis-related gene mRNA, inhibit the growth of human hepatocellular carcinoma SMMC-7721 cells and induce apoptosis, and play a therapeutic role in the treatment of liver cancer.