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目的探讨转录激活因子4(activating transcription factor 4,ATF4)在许多肿瘤细胞的缺氧区被检测到呈高表达,并且与肿瘤的恶性程度相关。核糖体蛋白L41(ribosomal protein L41,RPL41)能够诱导肿瘤细胞死亡和细胞周期停滞,在调节ATF4的水平方面发挥着重要作用。本研究探讨ATF4和RPL41在上皮性卵巢癌组织中的表达及其与临床病理学特征的关系。方法利用免疫组化技术SP法分别检测中国医科大学附属盛京医院病理科2000-01-01-2015-12-31470例上皮性卵巢癌、42例交界性卵巢肿瘤、27例良性卵巢肿瘤和46例正常卵巢组织切片及芯片中ATF4和RPL41的表达情况。结果 ATF4在上皮性卵巢癌组织中阳性表达率为86.60%(407/470),明显高于正常卵巢与良性卵巢肿瘤组的39.73%(29/73)及交界性卵巢肿瘤组的73.81%(31/42),差异有统计学意义,P值分别为<0.001和0.024。ATF4在上皮性卵巢癌组织中的表达明显高于其他组,并且与上皮性卵巢癌的组织病理学分级、临床分期、组织学类型及淋巴结转移均显著相关,P<0.05。RPL41在正常卵巢与良性卵巢肿瘤组中的阳性表达率为82.19%(60/73),明显高于交界性卵巢肿瘤的54.76%(23/42)及上皮性卵巢癌的37.45%(176/470),差异有统计学意义,P值分别为0.002和<0.001。RPL41在正常卵巢及卵巢良性肿瘤组织中的表达明显高于其他组;在上皮性卵巢癌中与淋巴结转移有关,P=0.016;但与组织病理学分级、临床分期及组织学类型均无关,P>0.05。Spearman相关性分析显示,ATF4和RPL41在上皮性卵巢癌组织中呈中等程度相关,r=-0.508。结论 ATF4表达升高和RPL41表达降低可能在上皮性卵巢癌的发生、分化进展过程中起重要作用。
Objective To investigate the expression of activating transcription factor 4 (ATF4) in a large number of tumor cells in hypoxia region, which is highly correlated with the degree of malignancy. Ribosomal protein L41 (RPL41), which can induce tumor cell death and cell cycle arrest, plays an important role in regulating the level of ATF4. This study was to investigate the expression of ATF4 and RPL41 in epithelial ovarian cancer and its relationship with clinicopathological features. Methods Immunohistochemical SP method were used to detect the pathological department of Shengjing Hospital Affiliated to China Medical University 2000-01-01-2015-12-31470 cases of epithelial ovarian cancer, 42 cases of borderline ovarian tumors, 27 cases of benign ovarian tumors and 46 Cases of normal ovarian tissue sections and chip ATF4 and RPL41 expression. Results The positive rate of ATF4 in epithelial ovarian cancer was 86.60% (407/470), which was significantly higher than that in normal ovary and benign ovarian tumor (39.73%, 29/73) and in borderline ovarian tumor (73.81%, 31 / 42), the difference was statistically significant, P values were <0.001 and 0.024. The expression of ATF4 in epithelial ovarian cancer tissues was significantly higher than that in other groups, and was significantly correlated with histopathological grade, clinical stage, histological type and lymph node metastasis of epithelial ovarian cancer (P <0.05). The positive rate of RPL41 in normal ovarian and benign ovarian tumors was 82.19% (60/73), which was significantly higher than that in borderline ovarian tumors (54.76%, 23/42) and epithelial ovarian cancer (37.45%, 176/470 ), The difference was statistically significant, P values were 0.002 and <0.001. The expression of RPL41 in normal ovarian and benign ovarian tumors was significantly higher than that in other groups. The expression of RPL41 was correlated with lymph node metastasis in epithelial ovarian cancer (P = 0.016), but not with histopathological grade, clinical stage and histological type > 0.05. Spearman correlation analysis showed that ATF4 and RPL41 were moderately correlated in epithelial ovarian cancer tissues, r = -0.508. Conclusion The increased expression of ATF4 and the decreased expression of RPL41 may play an important role in the occurrence and differentiation of epithelial ovarian cancer.