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目的:研究在固定时间和频率下,矩形波形低频脉冲磁场(LF-PMF)对大鼠心肌微血管内皮细胞(CMECs)迁移和NO分泌能力的影响。方法:实验分为4组(对照组,1.0MT组,1.4MT组,1.8MT组)。对照组不加磁场干预,其余各组分别在频率为15Hz,磁场强度分别为1.0MT、1.4MT和1.8MT,时间为4h/d,连续照射3d的条件下,用三角波形作用离体培养的大鼠CMECs。利用transwell检测细胞迁移能力,硝酸还原酶法检测CMECs培养液中NO含量的变化。结果:1.0MT组,1.4MT组和1.8MT组LF-PMF迁移能力与对照组相比均有不同程度提高[(24.40±5.12)(个/视野)vs(22.00±3.87)(个/视野),P<0.05;(31.40±3.81)(个/视野)vs(22.00±3.87)(个/视野),P<0.05;(37.40±4.01)(个/视野)vs(22.00±3.87)(个/视野),P<0.01]。1.0MT组,1.4MT组和1.8MT组LF-PMF的NO分泌能力与对照组相比均有提高[(25.26±1.06)(μmol/L)vs(19.18±2.88)(μmol/L),P<0.05;(29.44±1.10)(μmol/L)vs(19.18±2.88)(μmol/L),P<0.05;(39.52±1.25)(μmol/L)vs(19.18±2.88)(μmol/L),P<0.01]。结论:低频脉冲磁场磁场对CMECs的生物学作用与磁场的强度有一定关系,1.4M和1.8MT组LF-PMF促进细胞迁移,使NO分泌能力能力提高。
Objective: To study the effect of LF-PMF on the migration and NO secretion of myocardial microvascular endothelial cells (CMECs) in a fixed time and frequency. Methods: The experiment was divided into 4 groups (control group, 1.0MT group, 1.4MT group, 1.8MT group). The control group without magnetic field intervention, the rest of the group were in the frequency of 15Hz, magnetic field strength were 1.0MT, 1.4MT and 1.8MT, the time of 4h / d, continuous irradiation 3d conditions, the role of triangular wave in vitro culture Rat CMECs. The transwell assay was used to detect the migration of cells. Nitric acid reductase method was used to detect the change of NO content in CMECs culture medium. RESULTS: The migration of LF-PMF in 1.0MT group, 1.4MT group and 1.8MT group were all improved to some extent compared with the control group [(24.40 ± 5.12) vs (22.00 ± 3.87) , P <0.05; (31.40 ± 3.81) vs (22.00 ± 3.87) / area, P <0.05; (37.40 ± 4.01) vs (22.00 ± 3.87) Field of view), P <0.01]. The NO secretion capacity of LF-PMF in 1.0MT, 1.4MT and 1.8MT groups was significantly higher than that in control group [(25.26 ± 1.06) μmol / L vs 19.18 ± 2.88 μmol / L, P (P <0.05); (29.44 ± 1.10) μmol / L vs 19.18 ± 2.88 μmol / L, P <0.05; 39.52 ± 1.25μmol / L vs 19.18 ± 2.88μmol / , P <0.01]. CONCLUSION: The biological effect of low-frequency pulsed magnetic field on CMECs has a certain relationship with the strength of magnetic field. LF-PMF in 1.4M and 1.8MT group can promote cell migration and increase NO secretion capacity.