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目的通过探讨大鼠不同采血部位常见生化指标的差异性,为毒理学研究提供客观、真实、准确的检测结果。方法比较不同采样条件和采样方式获得的大鼠血清中丙氨酸转氨酶和天冬氨酸转氨酶活性。结果大鼠不同部位采样丙氨酸转氨酶和天冬氨酸转氨酶检测结果均有较大的差异。结论规范大鼠血清的采样部位和采样方式,开展全程的质量控制是获得准确实验结果的重要保证。
Objective To explore the difference of common biochemical indexes of different blood sampling sites in rats and provide objective, true and accurate test results for toxicology research. Methods The serum alanine aminotransferase (ALT) and aspartate aminotransferase (ALT) activities of rats were compared under different sampling conditions and sampling methods. Results The results of alanine aminotransferase and aspartate aminotransferase in different parts of rats were significantly different. Conclusion It is an important guarantee to obtain accurate test results by standardizing the sampling site and sampling method of rat serum and carrying out quality control throughout the whole process.