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以提高古蜡梅的组织培养和试管苗移栽及栽培效果为目的,用嘉定钱门塘的古蜡梅为起始材料,进行了无菌苗诱导、快速繁殖、生根和移栽试验。结果表明,1/2MS作为基本培养基明显优于Read、WPM、N6;诱导培养基中添加2.0mg/L2iP、0.2mg/LIAA、0.5mg/LGA3和1.5-5.0mg/LPVP可提高古蜡梅的繁殖系数和抑制外植体褐化;适当降低快繁培养基中的激素浓度可以防止玻璃苗的发生;添加0.5mg/LIBA和0.05mg/LNAA可使古蜡梅组培苗的生根率在75%左右。试管苗移栽前进行1-2d的炼苗和移栽后2周内保湿防晒可以提高成活率;抑制杂草可以促进试管苗健壮生长;控制地下水位可以提高试管苗的根系活力。
In order to improve the tissue culture of P. przewalskii and the effect of transplanting and planting test-tube seedlings, the experiment was conducted using the P. chinensis from Jiading Qianmen pond as the starting material to induce, rapid propagation, rooting and transplanting. The results showed that 1 / 2MS as the basic medium was significantly better than Read, WPM, N6; induction medium added 2.0mg / L2iP, 0.2mg / LIAA, 0.5mg / LGA3 and 1.5-5.0mg / The propagation coefficient and the inhibition of explant browning; Appropriate to reduce the hormone concentration in fast propagation medium can prevent the occurrence of glass seedlings; Adding 0.5mg / LIBA and 0.05mg / About 75%. Test tube seedlings 1-2 days before transplanting and plasticizing transplanting 2 weeks after transplanting moisturizing sunscreen can improve the survival rate; weed suppression can promote the robust growth of plantlets; control of groundwater level can increase the vitality of test-tube plantlets.