[目的]研究不育枸杞花药营养物质代谢与花粉败育的关系,为枸杞不育系花粉败育机制研究提供理论依据。[方法]运用半薄切片和细胞化学染色技术,分别进行多糖(含淀粉粒)、脂类和蛋白质的染色鉴定,对枸杞不育系和可育系花药营养物质积累和分布状况进行观察和比较分析。[结果]不育系与可育系花药半薄切片经组织化学染色后,多糖PAS反应呈阳性,红色,淀粉粒为红色颗粒。脂类苏丹黑B染色呈阳性,黑色颗粒。蛋白质考马斯亮蓝染色呈阴性。表明枸杞不育系与可育系一样,花药发育过程中积累储存的营养物质是淀粉粒和脂滴,没有储藏形式的蛋白质。与可育系相比,不育系花药减数分裂后,淀粉粒在药隔薄壁组织中骤减,表皮和药室内壁中的淀粉粒也大幅减少,绒毡层没有脂质积累;绒毡层和四分孢子先后发生液泡化、进入解体过程。[结论]可育系药隔维管束多糖营养供应充足,绒毡层细胞多糖转化、合成等活动旺盛。而不育系药隔多糖供应在减数分裂后急剧减少,使得绒毡层细胞多糖含量减少,导致绒毡层细胞多糖转化、合成脂质等营养物质的机制发生紊乱。由此引发了绒毡层提前进入细胞程序性死亡过程,四分孢子因营养匮乏,最终解体。 [Objective] The research aimed to study the relationship between anther nutrient metabolism and pollen abortion in sterile Lycium barbarum L., and provide the theoretical basis for the research on the pollen abortion mechanism of the sterile line of Lycium barbarum. [Method] Semi-thin sections and cytochemical staining techniques were used to identify the polysaccharides (including starch granules), lipids and proteins, and to observe and compare the accumulation and distribution of nutrients in Lycium barbarum L. and sterile anther. analysis. [Result] The semi-thin section of sterile anther and fertile anther had histochemical staining, the polysaccharide PAS reaction was positive, red and the starch granules were red granules. Lipid Sudan Black B stained positive, black particles. Protein Coomassie blue staining was negative. The results showed that, like the fertile line, the Lycium barbarum CMS is an accumulation of starch and lipid droplets accumulated during anther development, and no protein in storage form. Compared with fertile lines, after anther meiosis of sterile lines, the starch granules abruptly decreased in the parenchyma parenchyma, the starch granules in the epidermis and the inner wall of the drug chamber also decreased significantly, and there was no lipid accumulation in the tapetum. Felt layer and tetradospores have occurred vacuoles, into the disintegration process. [Conclusion] There was sufficient nutrition supply of polysaccharide in the vascular bundle of fertile lines, vigorous cell transformation and synthesis of tapetal cells. However, the supply of drug-resistant polysaccharide in CMS decreased sharply after meiosis, which resulted in the decrease of polysaccharide content in tapetal cells, leading to the disturbance of the mechanism of polysaccharide transformation in tapetal cells and the synthesis of lipid and other nutrients. As a result, the tapetum entered the process of programmed cell death in advance, and the quadruple spore was finally disintegrated due to lack of nutrition.