Molecularly imprinted polymers(MlPs) were applied as molecular recognition elements to an electrochemical sensor for cinchonidine(CD).A kind of MIP was synthesized with cinchonidine as template,modified rosin(ethylene glycol maleic rosinate acrylate) containing the skelcton of phenanthrene rings as cross-linker and methylacrylic acid as functional monomer.MIP membrane was prepared on a glassy carbon electrode for the determination of CD via free radical polymerization method.Electrochemical impedance spectroscopy(EIS) and cyclic voltammetry(CV) were used to characterize the membrane electrochemical behavior in electrode fabrication process.The experimental conditions were discussed.Under optimum conditions,it was found that the response of peak currents was linear to the concentration of CD in a range of 0.013--2.26 mmol/L.The detection limit for CD is 1 μmol/L,the relative standard deviation for 100 μ mol/L CD is 1.34％ and the incubation time is 2 min.The sensor was applied to the determination of CD in urine samples with satisfactory results.