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背景:巴曲酶是目前比较公认的治疗缺血性脑血管病的理想药物之一,被广泛地应用于临床,因此对其在脑缺血再灌注损伤中的保护作用进行深入认识很有必要。目的:探讨巴曲酶对大鼠局灶性脑缺血再灌注后血小板活化因子(PAF)水平及PAF受体基因(PAF-RmRNA)表达的影响。设计:完全随机区组设计。地点和对象:实验于2004-03/12在哈尔滨医科大学附属第二医院科研中心完成。选择40只健康Wistar雄性大鼠,体质量200～250g,随机分为5组,每组8只。Ⅰ组:假手术组;Ⅱ组为生理盐水组:Ⅱa为缺血6h再灌注6h组,Ⅱb为缺血6h组;Ⅲ组为巴曲酶组:Ⅲa为缺血6h再灌注6h组,Ⅲb为缺血6h组。方法:线栓法建立大鼠大脑中动脉闭塞(MCAO)及再通模型。应用RT-PCR技术检测MCAO及再通后缺血半暗带皮质PAF受体基因表达,同时用ELISA检测对应血浆PAF值。主要观察指标:不同时间点各组缺血半暗带皮质PAFmRNA表达及血浆PAF值。结果:生理盐水组中再灌组及缺血组PAF值均明显升高,Ⅱa,Ⅱb分别为(1480±249)和(1052±199)ng/L,而PAF-RmRNA表达降低,分别为0.44±0.06和0.48±0.05,分别与对应假手术组比较非常显著性意义(P<0.01)。巴曲酶组中再灌注及缺血组PAF值均降低,为(848±80)和(743±105)ng/L,PAF-RmRNA表达增强(0.63±0.08和0.67±0.06),与对应生理盐?
BACKGROUND: Batroxobin is one of the most ideal drugs for the treatment of ischemic cerebrovascular disease and is widely used in clinical practice. Therefore, it is necessary to understand its protective effect in cerebral ischemia-reperfusion injury . Objective: To investigate the effect of batroxobin on the level of platelet activating factor (PAF) and the expression of PAF receptor gene (PAF-R mRNA) after focal cerebral ischemia-reperfusion in rats. Design: Completely random block design. Location and Subject: The experiment was performed at the Research Center of the Second Affiliated Hospital of Harbin Medical University from March to December 2004. Forty healthy Wistar male rats weighing 200-250 g were randomly divided into 5 groups with 8 rats in each group. Group Ⅰ: Sham operation group; Group Ⅱ: saline group Ⅱa: ischemia-reperfusion 6h after ischemia 6h, ischemia-reperfusion 6h group Ⅱb; batroxobin group Ⅲ: ischemia reperfusion 6h group Ⅲb 6h group for ischemia. Methods: The middle cerebral artery occlusion (MCAO) and recanalization model were established by thread occlusion. RT-PCR was used to detect the gene expression of PAF receptor in ischemic penumbra and cortex of MCAO and recanalization, and the corresponding plasma PAF was detected by ELISA. MAIN OUTCOME MEASURES: PAF mRNA expression in the ischemic penumbra and plasma PAF at different time points. Results: PAF in reperfusion group and ischemia group were significantly increased in the saline group (1480 ± 249 and 1052 ± 199 ng / L, respectively), while the expression of PAF-R mRNA was decreased ± 0.06 and 0.48 ± 0.05, respectively, compared with the corresponding sham operation group was very significant (P <0.01). PAF in reperfusion and ischemic groups of Batroxobin group were significantly lower (848 ± 80 and 743 ± 105 ng / L, PAF-R mRNA were significantly increased (0.63 ± 0.08 and 0.67 ± 0.06, respectively, salt?