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作者构建了日本脑炎病毒（Ｊａｐａｎｅｓｅｅｎｃｅｐｈａｌｉｔｉｓｖｉｒｕｓ，ＪＥＶ）前膜蛋白囊膜蛋白（ｐｒＭＥ）和乙型肝炎病毒（ｈｅｐａｔｉｔｉｓＢｖｉｒｕｓ，ＨＢＶ）前Ｓ蛋白（ｐｒｅＳ）的真核表达载体，分别命名为ｐｃＤＮＡ３Ｅ和ｐＳＧ５ｐｒｅＳ。等量混合这两种质粒，转染ＣＯＳ７细胞进行瞬间表达，应用单克隆抗本ＥＬＩＳＡ法检测特异性抗原。结果表明，单独及混合转染的细胞培养上清中均有特异性抗原表达。本项研究为进一步发展新型日本脑炎病毒和乙型肝炎病毒核酸疫苗打下了基础，也初步显示了核酸疫苗技术在联合疫苗研制中的应用前景
The authors constructed eukaryotic expression vectors of Japanese encephalitis virus (JEV) pre-membrane protein-envelope protein (prM-E) and hepatitis B virus (preS) preS protein and named as pcDNA3 E and pSG5 preS. The same amount of mixing these two plasmids, transiently transfected COS 7 cells, the application of monoclonal anti-ELISA detection of specific antigens. The results showed that both the single and mixed transfected cell culture supernatant had specific antigen expression. This study laid the foundation for the further development of new Japanese encephalitis virus and hepatitis B virus nucleic acid vaccine and also initially showed the application prospect of nucleic acid vaccine technology in the development of combined vaccine