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目的对我室构建恶性疟原虫海南株（FCC／HN）红内期cDNA表达文库阳性克隆（FMPf01）进行分析研究，确定其性质。方法通过计算机软件和免疫印迹法分析FMU01的减基构成、同源性及编码多肽的免疫反应性。结果核苷酸序列分析显示其A＋T构成比高于G＋C，为3．14：1，与基因资料库中疟原虫基因的同源性较低，其最高值为63．8％，Westernblot分析证实其编码多肽可被抗疟原虫免疫血清识别，反应性较强。结论FMPf01编码多肽有望成为疟疾免疫学诊断和疫苗的候选抗原。
OBJECTIVE To analyze the positive clones (FMPf01) of cDNA library of Plasmodium falciparum Hainan (FCC / HN) during the construction of our laboratory and to determine their properties. Methods The subtractive composition, homology and immunoreactivity of the encoded polypeptide were analyzed by computer software and Western blotting. Results The nucleotide sequence analysis revealed that the A + T ratio was 3.14: 1, which was lower than that of the G + C gene. The highest homology was 63.8% with Western blot analysis The encoded polypeptide can be recognized by anti-malaria immune sera with high reactivity. Conclusion FMPf01 encoding polypeptide is expected to be a candidate antigen for malaria immunological diagnosis and vaccine.