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目的观察PDGFB链基因三链形成寡核苷酸(triplexformingoligonucleotide,TFO)对C6胶质瘤细胞增殖和凋亡的影响。方法应用流式细胞技术观察TFO对C6胶质瘤细胞PDGFB、PCNA表达的影响,应用流式细胞技术观察TFO对C6胶质瘤细胞凋亡的影响。结果TFO对C6胶质瘤细胞PDGFB、PCNA的表达有明显抑制作用,而且抑制作用存在浓度依赖性。TFO有明显诱导C6胶质瘤细胞细胞凋亡作用,而且诱导作用存在浓度依赖性。结论TFO抑制C6胶质瘤细胞细胞增殖,同时诱导C6胶质瘤细胞细胞凋亡。
Objective To observe the effect of triplexformingoligonucleotide (PDO) triplex forming oligonucleotide (TFO) on the proliferation and apoptosis of C6 glioma cells. Methods The effect of TFO on the expression of PDGFB and PCNA in C6 glioma cells was observed by flow cytometry. The effect of TFO on the apoptosis of C6 glioma cells was observed by flow cytometry. Results TFO had a significant inhibitory effect on the expression of PDGFB and PCNA in C6 glioma cells, and the inhibitory effect was concentration-dependent. TFO significantly induced apoptosis of C6 glioma cells, and the induction of concentration-dependent. Conclusion TFO inhibits the proliferation of C6 glioma cells and induces the apoptosis of C6 glioma cells.