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在烟属中,人们已成功地应用胚珠培养获得一些种间组合的杂种,防止胚珠在杂交传粉后数日内死亡。但波缘烟草(Nicotiana repanda W.)和普通烟草(N.tabacum L.)种间组合时,杂种籽苗都在发育早期死亡。据认为这是杂种不育性的表现。但是,如能消除一小部分使杂种致死的雄性染色体组,便有可能得到杂种植株。虽然黄花烟草(N.rustca)和普通烟草间杂交组合没有表现出杂种不育性,但 Shizukttda 等(1983)给黄花烟草的单倍体染色体加进普通烟草的不完全染色体成功地育出“部分杂种”。他们先用电离辐射破坏花粉粒的染色体后进行传粉,然后离体培养杂交胚珠。本研究采用上述技术以探索克服波缘烟草和普通烟草杂种不育的可能性。
In tobacco, one has successfully applied ovule culture to obtain some cross-species hybrids that prevent the ovule from die within days after hybridization. However, when interspecific combinations of Nicotiana repanda W. and N. tabacum L. occur, hybrid seedlings die early in their development. It is considered that this is the performance of hybrid sterility. However, it is possible to obtain hybrid plants if a small fraction of the male chromosomes lethal to hybrids can be eliminated. Although cross combinations of N. rustca and N. tabacum do not exhibit hybrid sterility, Shizukttda et al. (1983) successfully introduced the haploid chromosomes of N. tabacum into the Nicotiana tabacum chromosomes with incomplete chromosomes Hybrid ". They first pollinated the pollen grains by ionizing radiation and then cultured the hybrid ovules in vitro. In this study, the above techniques were used to explore the possibility of overcoming the sterility of crossed tobacco and common tobacco.