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目的探讨VDR基因多态性与2型糖尿病肾病的相关性.方法应用TaqMan荧光探针技术,对235例2型糖尿病患者其中正常白蛋白尿(DKD0)组82例,微量白蛋白组(DKD1)组66例,大量白蛋白尿组(DKD2)组87例和86例健康对照者(NGT)组的VDR基因BsmⅠ、ApaⅠ位点SNP进行检测,比较和分析各组间基因型频率和等位基因频率以及相关临床资料.结果 (1)BsmⅠ位点GG、GA、AA基因型频率分别为0.931、0.066、0.003,等位基因G和A频率分别为0.964、0.036;ApaⅠ位点CC、CA、AA因型频率分别0.536、0.371、0.093,等位基因C和A频率分别为0.721、0.279;(2)DKD2组ApaⅠ位点C等位基因频率(0.770)高于DKD0组患者(0.671),A等位基因频率(0.230)低于于DKD0组患者(0.329),P<0.05;(3)Logistic回归分析表明VDR基因BsmⅠ位点GG基因型可能是T2DKD发生的独立保护因素(OR=0.159,P<0.05),但与DKD进展无关;ApaⅠ位点SNP与T2DKD的发生及进展均无关(P>0.05).25(OH)D缺乏或不足是T2DKD进展的危险因素(OR=1.957,P<0.05).结论 VDR基因BsmⅠ位点GG基因型可能是T2DKD发生的独立保护因素,但与DKD进展无关;ApaⅠ位点SNP与T2DKD的发生及进展均无关.
Objective To investigate the relationship between polymorphism of VDR gene and type 2 diabetic nephropathy.Methods A total of 82 patients with type 2 diabetes mellitus (DKD0), DKD1 (microalbumin group) Group B 66 cases, 87 cases of massive albuminuria group (DKD2) group and 86 cases of healthy control group (NGT) group were detected Bsm Ⅰ, Apa Ⅰ site SNP were compared and analyzed between the genotype frequencies and alleles Frequency and related clinical data.Results (1) The frequency of GG, GA, AA genotypes at BsmⅠ locus was 0.931,0.066,0.003, and the frequencies of allele G and A were 0.964,0.036 respectively. The ApaⅠ locus CC, CA, AA (2) The frequencies of C allele at ApaⅠ locus in DKD2 group (0.770) were higher than those in DKD0 group (0.671), A, etc. (3) Logistic regression analysis showed that GG genotype of VDR gene BsmI site may be an independent protective factor of T2DKD (OR = 0.159, P < 0.05), but not with the progression of DKD; SNP of ApaⅠwere not related to the occurrence and progression of T2DKD (P> 0.05) .25 (OH) D was deficient or deficient (OR = 1.957, P <0.05) .Conclusion GG genotype of BsmⅠin VDR gene may be an independent protective factor for the development of T2DKD, but it has no relation with the progression of DKD.The occurrence and progression of ApaⅠ SNP and T2DKD both Nothing to do