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用一种含头尾相连DHBVDNA双体的质粒体内转染2日龄芙蓉鸭，大多数鸭（86％）产生了短暂病毒血症。血清DHBs／preSAg和DHBVDNA于转染后第9天出现，第12～14天达峰值，第28天时多数转阴；少数鸭的病毒血症可持续50天以上。转染鸭肝组织中也检测到复制中间型DHBVDNA的存在。用转染鸭病毒血症期的血清作磷钨酸负染电镜观察，找到了完整的DHBV病毒颗粒，并且用此血清腹腔注射1日龄鸭，60％的鸭被感染成功，证明体内转染后有生物活性的DHBV病毒颗粒的产生。该研究方法的建立．对于研究DHBV变异株．DHBV基因结构与功能的关系等，均有一定理论意义及应用价值。
A 2-day-old lotus duck was transfect in vivo with a plasmid containing the head-to-tail linked DHBVDNA diabody, and most of the ducks (86%) developed transient viremia. Serum DHBs / preSAg and DHBVDNA appeared on the 9th day after transfection, reached the peak value on the 12th to the 14th day, and turned negative on the 28th day. The viremia of a few ducks could last more than 50 days. The presence of replicative intermediate DHBVDNA was also detected in transfected duck liver tissues. Using the serum transfection of duck viraemia period of tungstophosphoric acid negative staining electron microscopy, to find a complete DHBV virus particles, and the use of this serum intraperitoneal injection of 1-day-old duck, 60% of the ducks were infected successfully, in vivo transfection After the production of bioactive DHBV virus particles. The establishment of this research method. For studying DHBV variants. DHBV gene structure and function of the relationship, have a certain theoretical significance and application value.