动物实验及临床观察表明,流感嗜血杆菌、卡他尔球菌等革兰氏阴性菌产生的内毒素具有很强的促进异形细胞增殖和形成的能力,同分泌性中耳炎(OME)的发生、发展有关,但其确切机理尚不清楚。在以往对大鼠培养中耳上皮进行系列研究的基础上,应用自行创用的胶原下层(collagenousunderl Animal experiments and clinical observations have shown that endotoxins produced by Gram-negative bacteria such as Haemophilus influenzae and Enterococcus kalium have a strong ability to promote the proliferation and formation of isoforms and the occurrence and development of secretory otitis media (OME) However, the exact mechanism is not yet clear. Based on the previous studies on rat middle ear epithelium culture, the collagenousunderl