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选取来自浙江建德、安徽当涂、江苏太湖3个不同地理群体的河川沙塘鳢,采用3×3完全双列杂交方式进行配组,对9个组合F1子代的生长性状及遗传多样性进行比较分析。通过对2月龄和4月龄F1子代的生长性状进行对比分析发现,建德(♂)×当涂(♀)杂交组合的全长增加率、质量增加率分别为42.58%、190.79%,建德×当涂(正反交)组合的全长杂交优势率、体质量杂交优势率分别为17.88%、66.75%,均高于其他组合。不同地理群体间的杂交F1子代的全长增加率、质量增加率、杂交优势率均高于同一地理群体间的自繁F1子代。采用8个微卫星引物分析获得9个组合F1子代的遗传分化指数平均值为0.195,表明9个组合子代间存在遗传差异与分化,其期望杂合度为0.1493~0.7971,观测杂合度为0.1562~1.0000。双列杂交子代的杂合度和遗传多样性高于自繁子代,其中建德(♂)×当涂(♀)组合的等位基因数、多态信息含量值分别为3.9和0.486,均高于其他组合。综合生长性能和遗传多样性,初步选择建德(♂)×当涂(♀)为最佳杂交组合。
In this study, 3 × 3 complete diallel cross combinations were used for the breeding of the three populations from Jiande, Zhejiang, Dangtu, Jiangsu and Taihu Lake. The growth traits and genetic diversity For comparative analysis. Through the comparative analysis of growth traits of two-month-old and four-month-old F1 progenies, the full-length increase rate and mass increase rate of Jiande (♂) × Dao (♀) hybrid combination were 42.58%, 190.79% The prevalence of full-length hybridization and the prevalence of body weight hybridization were 17.88% and 66.75% respectively for Jiande × Dangdou (orthologous and reciprocal crossing) combinations, which were higher than other combinations. The F1 growth rate, the mass increase rate and the heterosis rate of hybrid F1 progenies among different geographic groups were higher than that of the F1 progenies of the same geographical group. The average genetic differentiation index of 9 F1 progeny combinations obtained by 8 microsatellite primers was 0.195, indicating that there were genetic differences and differentiation between the 9 progenies. The expected heterozygosity was 0.1493 ~ 0.7971 and the observed heterozygosity was 0.1562 ~ 1.0000. The heterozygosity and genetic diversity of the two-line hybrid progenies were higher than that of the self-progeny progenies. The allele number and polymorphism information content of Jiande (♂) × Dangtu (♀) combinations were 3.9 and 0.486, respectively, In other combinations. According to the comprehensive growth performance and genetic diversity, Jiande (♂) × Dangtu (♀) was selected as the best cross combination.