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为探讨多药耐药基因(mdt-1)与急性白血病(AL)的临床特征、免疫分型以及预后关系,用间接免疫荧光法和逆转录—多聚酶链式反应检测63例成人AL细胞mdr-l(即P-170和mRNA)表达。结果显示,复发和难治患者mdr-l表达阳性率明显高于初治患者,分别为50.0％、50.0％和21.3％(P<0.05)。在M_1、M_5和慢性粒细胞白血病急变患者mdr-l基因表达率较高,为50.0％、47.1％和85.7％(P尸<0.01)。Mdr-1基因表达与细胞CD_14表达有关(P<0.01)。Mdr-l表达增高患者,获得完全缓解的机会较少,且完全缓解期、生存期短。1年生存率mRNA表达阳性患者11.0％,mRNA表达阴性患者58.0％。提示mdr-1基因表达水平影响AL的预后,可做为评价AL的预后参数。
To explore the relationship between multidrug resistance gene (mdt-1) and acute leukemia (AL), clinical features, immunophenotype, and prognosis, 63 cases of adult human AL cell mdr- were detected by indirect immunofluorescence and reverse transcription-polymerase chain reaction. l (ie P-170 and mRNA) expression. The results showed that the positive rate of mdr-1 expression in relapsed and refractory patients was significantly higher than that in the untreated patients (50.0%, 50.0%, and 21.3%, respectively) (P<0.05). The rate of mdr-1 gene expression was significantly higher in M_1, M_5 and CML leukemia patients, 50.0%, 47.1%, and 85.7% (P corp. <0.01). The expression of Mdr-1 gene was related to the expression of cell CD14 (P<0.01). Patients with increased Mdr-1 expression had less chance of achieving complete remission, and had a complete remission period and a short survival period. One-year survival rate was 11.0% in patients with positive mRNA expression and 58.0% in patients with negative mRNA expression. It is suggested that the expression level of mdr-1 gene affects the prognosis of AL and can be used as a prognostic parameter of AL.