[目的]建立高效液相色谱法测定20%氯胺嘧草醚悬浮剂中有效成分的分析方法。[方法]使用Acclaim C_(18)色谱柱,以乙腈和水作为流动相(体积比80∶20),检测波长为245 nm,对氯胺嘧草醚进行定量分析。[结果]当氯胺嘧草醚的质量浓度为11.7~468 mg/L时,该方法的线性相关系数为1.0000,相对标准偏差为0.11%,平均回收率为99.09%。[结论]该方法具有较宽的线性范围,准确度、精密度好,操作方便,可以用作氯胺嘧草醚悬浮剂的定量分析。 [Objective] To establish an HPLC method for the determination of active ingredients in 20% CMX suspension. [Method] The analytical column of Acclaim C_ (18) was used with acetonitrile and water as mobile phase (volume ratio 80:20) and the detection wavelength was 245 nm. [Result] The linear correlation coefficient of this method was 1.0000, the relative standard deviation was 0.11% and the average recovery was 99.09% when the concentration of chlorimuron was 11.7 ~ 468 mg / L. [Conclusion] The method has wide linear range, good accuracy and precision, and is convenient to operate. It can be used as a quantitative analysis of the chloramphenicol suspension.