实时定量PCR具有灵敏、准确、高通量等优点,已广泛应用于基因表达分析。常规的实时定量PCR关键步骤是选择合适的内参基因进行校正。该文总结了模式植物番茄传统内参基因类型的研究现状,提出了新的内参基因挖掘方法,以期为番茄实时定量PCR标准化提供了重要的理论依据。 Real-time quantitative PCR has the advantages of sensitivity, accuracy, high throughput and has been widely used in gene expression analysis. Routine real-time quantitative PCR is the key step to select the appropriate internal reference gene for correction. This paper summarizes the current research status of traditional reference gene types in tomato plants and proposes a new internal reference gene mining method, which provides an important theoretical basis for the standardization of real-time PCR.