论文部分内容阅读
以萝卜３个不育系Ａ１、Ａ５、Ａ６与２个自交系５４、５５号配组的６个Ｆ１代及亲本为材料，分别在营养生长期取根、叶，生殖生长期取茎生叶、花蕾，对酯酶（ＥＳＴ）、α－淀粉酶（α－Ａｍｙｌａｓｅ）、细胞色素氧化酶（ＣＯＤ）、过氧化物酶（ＰＯＤ）进行比较研究。将各时期、各部位的同工酶酶带按Ｒｆ值大小排列，酯酶共有２１条带，分为快、中、慢３个区；随着植株生长，α－淀粉酶的３条酶带Ｒｆ值有增加趋势；ＣＯＤ共有１１条带，其中有Ｒｆ０．１３和０．１７两条保守酶带；ＰＯＤ共有８条带，有Ｒｆ０．１７一条保守酶带。Ｆ１代与双亲各种酶谱相比大多表现为双亲互补型，没有发现杂种酶带。
Six F1 progenies of the three CMS lines A1, A5, A6 and two inbred lines 54 and 55 and their parents were used as materials to take roots, leaves and reproductive growth stages of vegetative growth stages Leaf, flower buds, esterase (EST), α-Amylase, cytochrome oxidase (COD), peroxidase (POD) were compared. In each period, the isozymes of various parts of the enzyme bands were arranged according to the size of Rf. There were 21 bands of esterase, which were divided into three zones: fast, medium and slow. With the growth of plants, Rf value tends to increase. COD has 11 bands, of which there are two conserved bands of Rf0.13 and 0.17. There are 8 bands of POD and 1 band of Rf0.17. F1 generation and parents compared to the various zymograms compared to most of the parents showed complementary type, did not find hybrid enzyme band.