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目的 :研究视网膜中表达生长抑素mRNA神经元的发生和发育规律。方法 :原位杂交组织化学。结果 :胚胎第十三天 (E1 3 ) ,视网膜内层就可以检测到生长抑素mRNA阳性细胞。从E1 4到E1 7阳性细胞增加迅速 ,E1 7达到高峰 ,此时视网膜节细胞层大部分为生长抑素mRNA阳性细胞 ,细胞排列紧密。从E1 8开始 ,阳性细胞逐渐减少 ,细胞排列开始稀疏。出生后当 (PND0 )阳性细胞数显著下降。从PND0到PND1 5 ,阳性细胞继续减少 ,阳性细胞主要位于节细胞层。PND2 0时 ,阳性细胞的数目及分布均与成年动物相似 ,部分已迁移至内核层的内表面。细胞开始或停止表达生长抑素mRNA均是从视网膜中央开始 ,然后向周围。结论 :首次发现生长抑素基因在胚胎期的节细胞中一过性表达 ,提示生长抑素在视网膜节细胞的产生 ,分化以及视觉通路的形成与成熟有关。
Objective: To study the occurrence and development of somatostatin mRNA expression in the retina. Methods: In situ hybridization histochemistry. Results: On the 13th day of embryo (E1 3), somatostatin mRNA positive cells could be detected in the retina. From E1 4 to E1 7 positive cells increased rapidly, E1 7 reached its peak, at this time the majority of retinal ganglion cell somatostatin mRNA positive cells, cells arranged closely. Starting from E1 8, the number of positive cells gradually decreased and the arrangement of cells began to be sparse. After birth when (PND0) positive cells decreased significantly. From PND0 to PND1 5, the positive cells continued to decrease, and the positive cells mainly located in the ganglion cell layer. At PND20, the number and distribution of positive cells were similar to those in adult animals and some had migrated to the inner surface of the inner nuclear layer. Cells begin or stop expressing somatostatin mRNA from the center of the retina and then to the surroundings. Conclusion: We found for the first time that somatostatin gene transiently expressed in embryonic ganglion cells, suggesting that somatostatin may be involved in the generation and differentiation of retinal ganglion cells and the formation and maturation of visual pathways.