ＣＤ１４作为革兰氏阴性菌细胞壁脂多糖的受体，表达在吞噬细胞膜表面上。虽然已报道有几种脂多糖受体存在，但已充分证明ＣＤ１４为其主要受体。为了进一步研究ＣＤ１４在革兰氏阴性菌感染中的作用机理以及与其它受体之间的相互关系，拟通过灭活ＣＤ１４的研究方法以达此目的。为此，首先克隆了ＴＨＰ－１细胞的ＣＤ１４基因片段，通过测序已予确认，并已亚克隆于真核表达载体中，为进一步研究提供了可靠的物质基础。 CD14 acts as a receptor for Gram-negative bacterial cell wall lipopolysaccharide and is expressed on the surface of phagocyte membranes. Although several lipopolysaccharide receptors have been reported, CD14 has been well established as its major receptor. In order to further study the mechanism of CD14 in Gram-negative bacteria infection and its relationship with other receptors, it is proposed that this be done by inactivating CD14. To this end, we first cloned the CD14 gene fragment of THP-1 cells, confirmed by sequencing and subcloned into eukaryotic expression vector, providing a reliable material basis for further study.