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明日叶(Angelica keiskei Koidzumi)是伞形花科当归属植物,具有很高的药用价值。为解决生产上短时间快速获得大量明日叶种苗的相关技术,以明日叶叶片为外植体进行组织培养实验,直接诱导产生丛生芽并且得到再生植株,建立了明日叶叶片离体再生快速繁殖体系。结果表明,诱导丛生芽分化的适培养基是MS+1.0 mg·L-1 2,4-D+0.2 mg·L-1 6-BA,诱导率可高达100%;诱导生根的适培养基是MS+1.0 mg·L-1 NAA,诱导率可达90%,将生长良好的再生植株进行移栽,存活率可达86%。
Angelica keiskei Koidzumi is an umbrella plant belonging to the genus Umbelliferae and has high medicinal value. In order to solve the problem that a large number of seedlings of Tomorrow leaves are quickly obtained in a short period of time in production, tissue explants of Tomorrow leaves were used as explants to induce the shoots and obtain regenerated plants. system. The results showed that the suitable culture medium for induction of cluster bud differentiation was MS + 1.0 mg · L-1 2,4-D + 0.2 mg · L-1 6-BA with the induction rate as high as 100% MS + 1.0 mg · L-1 NAA, the induction rate of up to 90%, the growth of well-regenerated plants for transplanting, the survival rate of up to 86%.