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目的:研究脑力苏胶囊对血管性痴呆(VD)小鼠一般情况和GSH-Px表达的影响,探讨该方药对大脑的部份保护作用及其防治机制。方法:采用反复夹闭颈总动脉方法建立血管性痴呆小鼠模型。将造模成功后的小鼠随机分成5组:假手术组、模型对照组、西药治疗组、脑力苏胶囊高剂量组和脑力苏胶囊低剂量组。造模成功后,给药第11天观察小鼠一般情况变化,第14天后快速断头取脑,制成10%的组织匀浆液,4℃下离心,取血浆测定谷胱甘肽过氧化物酶(GHS-Px)活性的变化。结果:实验结果显示造模后的VD小鼠体重增长速度均有不同程度的降低,各治疗组小鼠的体重增加明显;治疗组GSH-PX水平与模型组比较均出现不同程度的升高,以脑力苏各组表现明显(P<0.01)。结论:脑力苏胶囊可能通过提高脑组织中GHS-Px活性,从而提高血管性痴呆小鼠的学习记忆能力。
Objective: To study the effects of Naoli Su capsule on the general situation and the expression of GSH-Px in vascular dementia (VD) mice and to explore the partial protective effects of this recipe on the brain and its prevention and treatment. Methods: The mouse model of vascular dementia was established by repeatedly clipping the common carotid artery. The mice were randomly divided into 5 groups: sham-operated group, model control group, western medicine treatment group, high-dose Na-liangsu capsule and low-dose Naolisu capsule. After the successful modeling, the general situation of the mice was observed on the 11th day of administration. After 14 days, the brain was rapidly decapitated to make a 10% tissue homogenate, centrifuged at 4 ° C, and the content of glutathione peroxides Changes in enzyme (GHS-Px) activity. Results: The results showed that the body weight growth rate of VD mice after modeling decreased to some extent, and the body weight of mice in each treatment group increased obviously. Compared with model group, the GSH-PX level in treatment group increased to some extent, The performance of each group showed significant brain (P <0.01). Conclusion: Naolisu capsule may improve learning and memory ability of vascular dementia mice by increasing GHS-Px activity in brain tissue.