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目的探讨micro RNA(miRNA)在外阴鳞状细胞癌组织中的表达及其临床意义。方法选择中国医科大学附属盛京医院妇产科2010年3月至12月诊断为外阴鳞状细胞癌并行手术治疗的3例患者的外阴癌新鲜组织做为观察组,观察组患者癌旁组织作为对照组。提取总RNA,经质量鉴定后进行荧光标记,利用miRNA芯片技术检测两组组织中miRNA的表达。结果通过对基因杂交芯片进行数据分析,从中共鉴定到430个miRNA差异表达,其中42个miRNA表达差异具有统计学意义(P<0.05),其中20个miRNA表达上调,22个miRNA表达下调。结论外阴鳞状细胞癌组织与癌旁组织中miRNA表达存在明显差异,从中筛选的差异表达miRNA(miR-99b、miR-211、miR-21)可能成为评估外阴癌发病风险的参考指标。
Objective To investigate the expression of microRNA (miRNA) in vulvar squamous cell carcinoma and its clinical significance. Methods Three cases of vulvar cancer fresh tissue diagnosed as vulvar squamous cell carcinoma with concurrent surgical treatment from March 2010 to December 2010 in Shengjing Hospital of China Medical University were selected as observation group and paracancerous tissues of observation group as Control group. The total RNA was extracted and identified by mass spectrometry. The miRNAs were detected by miRNA microarray. Results By analyzing the data of gene hybridization chips, 430 miRNAs were identified from the Chinese Communists. Among them, the expression of 42 miRNAs was statistically significant (P <0.05). Among them, 20 miRNAs were up-regulated and 22 miRNAs were down-regulated. Conclusion The miRNAs expression in vulvar squamous cell carcinoma tissues and paracancer tissues are significantly different. The differentially expressed miRNAs (miR-99b, miR-211, miR-21) screened from them may be used as a reference index to evaluate the risk of vulvar cancer.