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目的观察甲萘威对雌性大鼠的生殖毒性并初步探讨其机制。方法雌性SD大鼠经口染毒甲萘威,剂量为0、1.028、5.140、25.704mg·kg-1·d-1。阴道脱落细胞涂片法观察大鼠动情周期的变化;放射免疫法测定其血清雌二醇(E2)、孕酮(P4)水平;分光光度法测定其血清超氧化物歧化酶(SOD)、谷胱甘肽硫转移酶(GST)的活力以及丙二醛(MDA)、谷胱甘肽(GSH)的含量。结果各剂量甲萘威染毒组大鼠动情周期数明显低于对照组。染毒后15d大鼠动情各期出现变化,与对照组的差异有统计学意义(P<0.05,P<0.01)。25.704mg·kg-1·d-1组大鼠体重增长明显低于对照组。各剂量染毒组大鼠的多个脏器系数均明显降低。25.704mg·kg-1·d-1组大鼠血清中E2水平为(19.93±2.21)nmoll,1.028mg·kg-1·d-1组大鼠P4水平为(1.21±0.40)nmoll,与对照组[(28.76±6.12)、(0.63±0.39)nmolL]的差异均有统计学意义(P<0.05)。随染毒剂量增高,大鼠SOD活力在卵巢先降后升,在血清中略升后下降;MDA含量则呈在卵巢中渐升高、在血清中略升后降低趋势;GSH含量和GST活力在卵巢中呈先降后升趋势,但在血清中,GSH含量呈下降趋势,GST活力先上升后下降。结论甲萘威可致雌性大鼠动情周期紊乱及雌激素水平改变,对大鼠的抗氧化系统产生一定影响。
Objective To observe the reproductive toxicity of carbaryl in female rats and to explore its mechanism. Methods Female Sprague-Dawley rats were orally administered carbaryl at dosages of 0, 1.028, 5.140 and 25.704 mg · kg -1 · d -1. Vaginal exfoliated cell smears were used to observe the changes of estrous cycle. Serum estradiol (E2) and progesterone (P4) levels were measured by radioimmunoassay. The levels of serum superoxide dismutase (SOD), glutathione The activity of glutathione S-transferase (GST) and the contents of malondialdehyde (MDA) and glutathione (GSH) were determined. Results The doses of carbaryl in rats were significantly lower than those in the control group. There was a significant difference in estrus between the 15th day and the control group (P <0.05, P <0.01). The weight gain of rats in 25.704mg · kg-1 · d-1 group was significantly lower than that in control group. The multiple organ coefficients of rats exposed to various doses were significantly lower. The level of E2 in serum of 25.704mg · kg-1 · d-1 group was (19.93 ± 2.21) nmoll, and the level of P4 in 1.028mg · kg-1 · d-1 group was (1.21 ± 0.40) nmoll, The difference of [(28.76 ± 6.12) and (0.63 ± 0.39) nmolL] was statistically significant (P <0.05). With the increase of exposure dose, the activity of SOD in ovary decreased first and then decreased in ovary, and then decreased slightly in serum. The content of MDA increased gradually in ovary and slightly increased in serum, and then decreased in serum. The content of GSH and the activity of GST in ovary In the serum, GSH content showed a downward trend, GST activity increased first and then decreased. Conclusion Carbaryl can cause female rats estrous cycle disorders and estrogen levels, the antioxidant system of rats have an impact.