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目的观察α-促黑素(α-MSH)促进脂肪酸氧化中的信号传导通路。方法 C2C12成肌细胞分成4组:对照组、α-MSH组、H-89加α-MSH组和RpcAMP加α-MSH组,分别处理后测定脂肪酸氧化和腺苷酸活化蛋白激酶(AMPK)活性。用免疫印迹法分组观察磷酸化的乙酰辅酶A羧化酶(ACC)。结果α-MSH组与对照组相比较显著增加了脂肪酸氧化和AMPK活性;但cAMP抑制剂(RpcAMP)和蛋白激酶A(PKA)抑制剂(H-89)均阻断了α-MSH的上述作用。免疫印迹法显示,α-MSH增加ACC的磷酸化,但同样被上述抑制剂阻断。结论α-MSH主要通过cAMP-PKA-AMPK-ACC的途径增加脂肪酸氧化。
Objective To observe the signal transduction pathway of α-melanin (α-MSH) in fatty acid oxidation. Methods C2C12 myoblasts were divided into 4 groups: control group, α-MSH group, H-89 plus α-MSH group and RpcAMP plus α-MSH group, and the activity of fatty acid oxidation and AMPK . Phosphorylated acetyl-CoA carboxylase (ACC) was screened by immunoblotting. Results Compared with the control group, α-MSH increased fatty acid oxidation and AMPK activity. However, both cAMP inhibitor (RpcAMP) and protein kinase A (PKA) inhibitor (H-89) blocked the above effects of α-MSH . Western blotting showed that α-MSH increased ACC phosphorylation, but was also blocked by the above inhibitors. Conclusion α-MSH increases fatty acid oxidation mainly through cAMP-PKA-AMPK-ACC pathway.