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目的分析CCL7与IL-6、TGF-β1的关系及相互作用,探讨CCL7在慢性乙型肝炎(CHB)、肝纤维化发生发展中的作用及其机制。方法选择慢性乙型肝炎患者48例,其中轻度、中度和重度分别为18、24和6例,另选40例健康体检者作为对照组。取研究对象血清,用酶联免疫吸附法(ELISA)检测IL-6、TGF-β1、CCL7血清水平。结果相关分析发现CHB患者血清CCL7与IL-6、TGF-β1水平呈正相关(r值分别为0.335、0.322、0.288,P<0.05)。慢性乙型肝炎患者血清IL-6、TGF-β1、CCL7水平显著高于对照组(P<0.01)。结论 CCL7血清水平随肝脏炎症和纤维化的发生与进展呈进行性增高,可作为评估肝脏炎症及纤维化程度的重要指标之一。
Objective To analyze the relationship and interaction between CCL7 and IL-6 and TGF-β1 and to explore the role of CCL7 in the pathogenesis of chronic hepatitis B (CHB) and hepatic fibrosis. Methods Forty-eight patients with chronic hepatitis B were selected. Among them, mild, moderate and severe were 18, 24 and 6, respectively. Another 40 healthy subjects were selected as the control group. Serum samples were collected and serum levels of IL-6, TGF-β1 and CCL7 were detected by enzyme-linked immunosorbent assay (ELISA). Results Correlation analysis showed that there was a positive correlation between the level of serum CCL7 and the levels of IL-6 and TGF-β1 in patients with CHB (r = 0.335,0.322,0.288, P <0.05). Serum levels of IL-6, TGF-β1 and CCL7 in patients with chronic hepatitis B were significantly higher than those in controls (P <0.01). Conclusion The serum level of CCL7 increases progressively with the occurrence and progression of liver inflammation and fibrosis, which may be used as an important index to evaluate the degree of liver inflammation and fibrosis.