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以侵染生姜的茎腐病菌Pythium myriotylum Drechsler、姜瘟病菌Ralstonia solanacearum和南方根结线虫Meloidagrne incognila为指示病原物,比较了两种土壤处理剂棉隆和氯化苦的熏蒸毒力。采用密闭玻璃容器熏蒸,结合菌丝生长速率法测得棉隆和氯化苦对生姜茎腐病菌的EC50值分别为0.284和0.096 mg/L,EC90值分别为18.106和0.312 mg/L。通过密封盘内摇床振荡(25℃,45 r/min)培养(24 h),结合菌悬液比色法评价了两药剂对姜瘟病菌的毒力。结果表明:氯化苦的活性较高,EC50和EC90值分别为0.176和1.280 mg/L,而棉隆的毒力较低,其EC50和EC90值分别达1 984和5 701 mg/L。采用线虫悬浮液密闭熏蒸法处理48 h,发现棉隆对南方根结线虫的EC50和EC90值分别为0.175和0.634 mg/L,氯化苦毒力略低于棉隆,其EC50和EC90值分别为1.211和3.772 mg/L。
Pythium myriotylum Drechsler, Ralstonia solanacearum and Meloidagrne incognila were used as the indicated pathogens to inoculate ginger, and the fumigation virulence of two soil treatment agents, cotton and chloropicrin, was compared. The EC50 values of cotton bollworm and chloropicrin to Ginger stem rot were 0.284 and 0.096 mg / L, respectively, and the EC90 values were 18.106 and 0.312 mg / L, respectively, using airtight glass containers. The virulence of the two agents against Magnaporthe grisea was evaluated by bacterial suspension colorimetric assay (24 h at 25 ° C, 45 r / min) with shaking in a sealed plate. The results showed that chloropicrin had high activity with EC50 and EC90 values of 0.176 and 1.280 mg / L, respectively. However, the toxicity of cotton was low with EC50 and EC90 values of 1 984 and 5 701 mg / L, respectively. The results showed that the EC50 and EC90 values of cottonseed against Meloidogyne incognita were 0.175 and 0.634 mg / L, respectively, and the chloropicrin toxicity was slightly lower than that of cotton bollworm. EC50 and EC90 values were respectively 1.211 and 3.772 mg / L.