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进行ＭＸＲ７基因ｃＤＮＡ的克隆鉴定并检测其在人正常组织和肿瘤组织中的表达。方法以人肝癌组织的ｃＤＮＡ为模板，应用ＰＣＲ方法合成ＭＸＲ７基因ｃＤＮＡ，连接于融合蛋白表达载体ｐＧＥＸ－５Ｘ－１，构建成符合读框的融合基因；应用Ｎｏｒｔｈｅｒｎｂｌｏｔ对正常人１２种不同组织和术前做治疗的７例非肝脏肿瘤组织及３０例肝癌和癌旁肝组织、１２例正常肝组织中ＭＸＲ７基因的表达进行检测。结果ＭＸＲ７ｍＲＮＡ在人肝脏等１２种正常组织及７例其它部位肿瘤组织中均不表达；在人肝癌中呈高表达，而癌旁肝组织中呈低表达，其阳性表达率分别为７６．７％和１３．３％。结论ＭＸＲ７．基因在人肝癌组织中的高水平表达具有普遍性和特异性，提示ＭＸＲ７基因可以作为肝癌的生物学标志物。
The MXR7 cDNA was cloned and identified for detection in human normal tissues and tumor tissues. Methods The cDNA of human hepatocellular carcinoma was used as a template. The cDNA of MXR7 gene was synthesized by PCR and ligated into the fusion protein expression vector pGEX-5X-1 to construct an in-frame fusion gene. The Northern blot was applied to 12 different tissues and techniques of normal people. The expression of MXR7 gene was detected in 7 non-liver tumor tissues treated before treatment, 30 liver cancer tissues and adjacent liver tissue, and 12 normal liver tissues. RESULTS: MXR7 mRNA was not expressed in 12 normal tissues and 7 other tumor tissues in human liver; it was highly expressed in human hepatocellular carcinoma and was lowly expressed in adjacent liver tissue, with a positive expression rate of 76.7%. And 13.3%. Conclusion MXR7. The high level of gene expression in human hepatocellular carcinoma is universal and specific, suggesting that MXR7 gene can be used as a biological marker of liver cancer.