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目的观察糖尿病(diabetes mellitus,DM)大鼠胰岛素治疗后阴茎勃起功能的情况及阴茎海绵体细胞凋亡、Bcl-2和Bax的基因表达。方法成年雄性Wistar大鼠50只,腹腔内注射链尿佐菌素(STZ)建立糖尿病动物模型,成模后8周根据阴茎勃起功能的情况,随机分成未行胰岛素治疗组(DM组)和胰岛素治疗组。胰岛素治疗组大鼠治疗16周后,处死并取阴茎海绵体,测血糖及糖化血红蛋白,用原位末端标记法(TUNEL)检测细胞凋亡,western blot方法检测阴茎组织Bcl-2、Bax蛋白的表达。结果DM组大鼠阴茎勃起功能明显低于胰岛素组。胰岛素组血糖及糖化血红蛋白下降(P<0.001),DM组大鼠较胰岛素治疗组大鼠阴茎海绵体凋亡细胞数明显增多,Bax表达增强,Bcl-2表达减弱。结论DM大鼠胰岛素治疗后,阴茎海绵体细胞凋亡率减少,阴茎勃起功能改善,Bcl-2和Bax可能参与了DM大鼠阴茎海绵体细胞凋亡的基因调控。
Objective To observe the erectile dysfunction and the gene expression of Bcl-2 and Bax in penis corpus cavernosum after diabetes mellitus (DM) rats. Methods Fifty adult male Wistar rats were injected intraperitoneally with streptozotocin (STZ) to establish animal models of diabetes mellitus. Eight weeks after the model was established, according to the condition of penile erection, they were randomly divided into three groups: the untreated insulin group (DM group) and insulin therapy group. After 16 weeks of treatment, the rats in the insulin treatment group were sacrificed and their corpus cavernosum, blood glucose and glycosylated hemoglobin were measured. Apoptotic cells were detected by TUNEL. Western blot was used to detect the protein expressions of Bcl-2 and Bax expression. Results The erectile function of DM group was significantly lower than that of insulin group. In the insulin group, the blood glucose and HbA1c decreased (P <0.001). The number of apoptotic cells, the expression of Bax and the expression of Bcl-2 in the DM group were significantly higher than those in the insulin treatment group. Conclusion After insulin treatment in DM rats, the apoptosis rate of penile corpus cavernosum and penile erectile function are improved. Bcl-2 and Bax may be involved in the gene regulation of apoptosis in the penis of DM rats.