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目的 探讨联合应用粉防己碱 (tetrandrine,Tet)与甘草酸 (glycyrrhizinicacid ,Glz)抗肝纤维化的分子生物学机制。方法 采用四氯化碳皮下注射诱导大鼠肝纤维化模型 ,同时给予不同剂量 (5mg/kg、10mg/kg、2 0mg/kg体重 )Tet、(5 0mg/kg体重 )Glz及二者联合灌胃或腹腔注射 ,用RT PCR法检测肝组织Ⅰ型、Ⅲ型前胶原、c fos及c junmRNA表达。结果 (10mg/kg、2 0mg/kg体重 )Tet和Glz单独应用能够不同程度抑制肝纤维化大鼠肝组织Ⅰ型、Ⅲ型前胶原、c fos及c junmRNA表达 ,两种药物联合应用效果更为显著。结论 联合应用Tet与Glz能够更有效地在转录及其上游水平抑制肝脏胶原合成。
Objective To investigate the molecular mechanism of tetrandrine (Tet) and glycyrrhizinic acid (Glz) in preventing hepatic fibrosis. Methods The model of hepatic fibrosis induced by subcutaneous injection of carbon tetrachloride was induced in rats. At the same time, Tet (50mg / kg, 10mg / kg, 20mg / kg) Tet, Gastric or intraperitoneal injection. The expression of type Ⅰ and type Ⅲ procollagen, c fos and c jun mRNA in liver tissue were detected by RT-PCR. Results (10mg / kg, 20mg / kg body weight) Tet and Glz alone could inhibit the expression of type Ⅰ, type Ⅲ procollagen, c fos and c jun mRNA in hepatic tissue of rats with hepatic fibrosis to some extent. The combined effect of the two drugs was more For the obvious. Conclusion The combination of Tet and Glz can inhibit liver collagen synthesis more efficiently at the transcriptional and upstream levels.