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Rubuphenol (1),a new polyphenolic compound,was isolated together with the known ellagic acid (2) as new cell cycle inhibitors from Rubus aleaefolius Poir.through a bioassay-guided separation procedure and the structure of 1 was elucidated by spectroscopic method.Compounds 1 and 2 inhibited the cell cycle of tsFT210 cells at the G0/G1 phase respectively with the MIC values of 14.6 (M and 10.3 (M.