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将含有人乳头瘤病毒１６型（ＨＰＶ_（１６））Ｅ_６基因的重组质粒ＰＡＳ１－ＨＰＶ_（１６）Ｅ_６转染大肠杆菌ＡＲ１２０，在萘啶酮酸诱导下进行表达，表达产物经分离、纯化和鉴定获得一种分子量为１９０００的Ｅ_６表达蛋白。用纯化后的Ｅ６蛋白免疫ＢＡＬＢ／ｃ小鼠，免疫脾细胞与骨髓瘤细胞ＳＰ２／０－Ａｇ１４在ＰＥＧ_（４０００）作用下进行融合，经ＨＡＴ培养基筛选杂交瘤细胞株，甲基纤维素半固体培养基克隆，克隆筛选，ＥＬＩＳＡ检测和再克隆，得到一株持续稳定分泌抗ＨＰＶ_（１６）Ｅ_６蛋白特异性单克隆抗体的杂交瘤株ＲＡＣ_６。
The recombinant plasmid PAS1-HPV_ (16) E_6 containing human papillomavirus type 16 (HPV_ (16)) E_6 gene was transfected into Escherichia coli AR120 and expressed in the presence of nalidixic acid. The expressed product was isolated, purified and identified An E6 protein with a molecular weight of 19000 was obtained. The purified E6 protein was used to immunize BALB / c mice. The spleen cells were fused with myeloma cells SP2 / 0-Ag14 under the action of PEG 4000 and the hybridoma cell lines were screened by HAT medium. A solid culture medium clone, clonal selection, ELISA detection and re-cloning were performed to obtain a stable and stable hybrid cell line RAC_6 secreting monoclonal antibody against HPV16 (16) E_6 protein.