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人类许多肿瘤可产生其母组织所不能产生的蛋白,如激素物质(AGTH、ADH甲状旁腺素)、胎儿性抗原(GEA、αFP)以及酶类(硷性磷酸酶)。这些非正常性蛋白产物均能在肿瘤和患者血液中测到。也有些此类蛋白不进入血液而仅存于肿瘤内。肿瘤内胶原基质一般都认为来自宿主被侵犯组织中的纤维母细胞。然而本文发现乳腺硬癌的胶原基质来自硬癌细胞而非纤维母细胞。作者先用动物分别制备抗胶原蛋白和抗普罗林羟基酶(Prolyl hydroxylase为一种合成胶原纤维不可缺少的酶)的单价特异抗体,在免疫组织化学染色法下研究乳腺硬癌的手术标本。在32例中有30例于癌细胞中发现含有胶原蛋白和普罗林羟基酶,而在瘤基质中的纤维母细胞内却毫无发现;同样在正常乳腺、乳腺纤维囊性病
Many human tumors produce proteins that cannot be produced by their parent tissues, such as hormone substances (AGTH, ADH parathyroid hormone), fetal antigens (GEA, αFP), and enzymes (Phosphatase). These abnormal protein products can be detected in the tumor and the patient’s blood. Some of these proteins do not enter the blood but only in the tumor. Intratumoral collagen matrix is generally considered to be derived from fibroblasts in the host’s invaded tissue. However, it was found herein that the collagen matrix of breast hard cancer is derived from hard cancer cells but not fibroblasts. The authors first prepared monovalent anti-collagen and anti-Prolyl hydroxylase (Prolyl hydroxylase, an indispensable enzyme that synthesizes collagen fibers) in animals, and studied surgical specimens of hard cancer of the breast under immunohistochemical staining. Of the 32 cases, 30 were found to contain collagen and prolyl hydroxylase in cancer cells but not in fibroblasts in the tumor stroma; also in normal breast, breast fibrocystic disease