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目的:研究以红花药材为原料,采用罐组式逆流提取技术提取羟基红花黄色素A(HSYA)的最优工艺条件。方法:考察了阶段提取时间、提取温度、乙醇浓度、料液比(g.mL-1)等因素对提取效果的影响,选用L9(34)正交试验法对提取工艺进行优化研究,并与传统浸提法和药典所述方法进行了比较。结果:阶段提取时间和料液比两个因素对HSYA提取率有显著性影响,最佳提取工艺为用10倍药材质量的30%乙醇,在室温下每个阶段提取120 m in。在此最佳工艺条件下,HSYA提取率为1.56%,浸出物中HSYA纯度为6.06%。与传统浸提法和药典中所述的超声提取法相比,提取率分别提高6.12%和9.09%,纯度分别提高42.9%和27.0%。结论:罐组式逆流提取技术具有操作简便、溶剂用量少、提取率和纯度较高等优点,具有很大的工业化应用价值。
OBJECTIVE: To study the optimum conditions for the extraction of hydroxysafflor yellow A (HSYA) using the Hongcao medicinal materials as raw material and the tank group countercurrent extraction technique. Methods: The effects of extraction time, extraction temperature, ethanol concentration, g.mL-1 and other factors on the extraction efficiency were studied. The extraction process was optimized by L9 (34) orthogonal test, The traditional method of extraction and pharmacopoeia were compared. Results: The extraction rate of HSYA was significantly affected by the extraction time and the ratio of solid to liquid. The optimum extraction process was extraction with 120% ethanol at 30% ethanol and 120 m in each phase at room temperature. Under the optimum conditions, the extraction rate of HSYA was 1.56% and the purity of HSYA in the extract was 6.06%. Compared with the ultrasonic extraction method described in the traditional leaching method and pharmacopoeia, the extraction rate was increased by 6.12% and 9.09%, respectively, with the purity increased by 42.9% and 27.0% respectively. Conclusion: The tank group countercurrent extraction technology has the advantages of simple operation, less solvent consumption, higher extraction rate and purity, and has great industrial application value.