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目的研究地塞米松对哮喘小鼠调节激活正常T细胞表达和分泌细胞因子(RANTES)蛋白和mRNA表达的影响。方法将30只雌性BALB/c小鼠随机分为3组(每组10只):对照组、哮喘组、激素干预组,收集支气管肺泡灌洗液(BALF),行白细胞和嗜酸粒细胞(EOS)计数;酶联免疫吸附法(ELISA)测定BALF和血清中RANTES含量;肺组织切片HE染色,光镜下计数细胞总数和EOS百分比;部分行免疫组化染色检测肺组织RANTES蛋白;用原位杂交法检测肺组织RANTESmRNA表达。结果哮喘组白细胞总数、EOS百分比、RANTES浓度明显升高(P<0.01),干预组明显低于哮喘组(P<0.01);哮喘组肺组织RANTES蛋白及mRNA表达显著高于对照组(P<0.01),主要表达于上皮细胞;干预组肺组织RANTES蛋白及mRNA表达显著低于哮喘组(P<0.01)。结论地塞米松可抑制RANTES表达和活性而发挥抗EOS炎症作用,这可能是其控制哮喘发病的重要机制之一。
Objective To investigate the effect of dexamethasone on the expression of activated normal T cells and the expression of RANTES protein and mRNA in asthmatic mice. Methods Thirty female BALB / c mice were randomly divided into three groups (n = 10): control group, asthma group, hormone intervention group, bronchoalveolar lavage fluid (BALF), leukocyte and eosinophil EOS). The contents of RANTES in BALF and serum were determined by enzyme-linked immunosorbent assay (ELISA). HE staining was used to determine the total number of cells and percentage of EOS in lung tissue sections. RANTES protein was detected by immunohistochemistry in some sections. The hybridization method was used to detect the expression of RANTES mRNA in lung tissue. Results The total number of leukocytes, the percentage of EOS and the concentration of RANTES in asthma group were significantly higher than those in asthma group (P <0.01). The expression of RANTES protein and mRNA in asthmatic group were significantly higher than those in control group (P < 0.01). The expression of RANTES protein and mRNA in the intervention group was significantly lower than that in the asthma group (P <0.01). Conclusion Dexamethasone can inhibit the expression and activity of RANTES and play an anti-EOS inflammatory effect, which may be one of its important mechanisms to control the pathogenesis of asthma.